844 research outputs found
Kinect Range Sensing: Structured-Light versus Time-of-Flight Kinect
Recently, the new Kinect One has been issued by Microsoft, providing the next
generation of real-time range sensing devices based on the Time-of-Flight (ToF)
principle. As the first Kinect version was using a structured light approach,
one would expect various differences in the characteristics of the range data
delivered by both devices. This paper presents a detailed and in-depth
comparison between both devices. In order to conduct the comparison, we propose
a framework of seven different experimental setups, which is a generic basis
for evaluating range cameras such as Kinect. The experiments have been designed
with the goal to capture individual effects of the Kinect devices as isolatedly
as possible and in a way, that they can also be adopted, in order to apply them
to any other range sensing device. The overall goal of this paper is to provide
a solid insight into the pros and cons of either device. Thus, scientists that
are interested in using Kinect range sensing cameras in their specific
application scenario can directly assess the expected, specific benefits and
potential problem of either device.Comment: 58 pages, 23 figures. Accepted for publication in Computer Vision and
Image Understanding (CVIU
Using the CRISPR/Cas9 system to understand neuropeptide biology and regulation
Funding was provided by a Wellcome Trust ISSF starting grant (105625/Z/14/Z), Medical Research Scotland (PhD-719-2013), GW Pharmaceuticals (PhD-719-2013 - S.5242.001) and the BBSRC (BB/J012343/1).Peer reviewedPublisher PD
Hand Tracking based on Hierarchical Clustering of Range Data
Fast and robust hand segmentation and tracking is an essential basis for
gesture recognition and thus an important component for contact-less
human-computer interaction (HCI). Hand gesture recognition based on 2D video
data has been intensively investigated. However, in practical scenarios purely
intensity based approaches suffer from uncontrollable environmental conditions
like cluttered background colors. In this paper we present a real-time hand
segmentation and tracking algorithm using Time-of-Flight (ToF) range cameras
and intensity data. The intensity and range information is fused into one pixel
value, representing its combined intensity-depth homogeneity. The scene is
hierarchically clustered using a GPU based parallel merging algorithm, allowing
a robust identification of both hands even for inhomogeneous backgrounds. After
the detection, both hands are tracked on the CPU. Our tracking algorithm can
cope with the situation that one hand is temporarily covered by the other hand.Comment: Technical Repor
Recombinase mediated cassette exchange into genomic targets using an adenovirus vector
Recombinase mediated cassette exchange (RMCE) is a process in which site-specific recombinases exchange one gene cassette flanked by a pair of incompatible target sites for another cassette flanked by an identical pair of sites. Typically one cassette is present in the host genome, whereas the other gene cassette is introduced into the host cell by chemical or biological means. We show here that the frequency of cassette exchange is dependent on the relative and absolute quantities of the transgene cassette and the recombinase. We were able to successfully modify genomic targets not only by electroporation or chemically mediated gene transfer but also by using an adenovirus vector carrying both the transgene cassette to be inserted and the recombinase coding region. RMCE proceeds efficiently in cells in which the adenovirus vector is able to replicate. In contrast, insufficient quantities of the transgene cassette are produced in cells in which the virus cannot replicate. Additional transfection of the transgene cassette significantly enhances the RMCE frequency. This demonstrates that an RMCE system in the context of a viral vector allows the site directed insertion of a transgene into a defined genomic site
Decoherence and Entropy Production in Relativistic Nuclear Collisions
Short thermalization times of less than 1 fm/c for quark and gluon matter
have been suggested by recent experiments at the Relativistic Heavy Ion
Collider (RHIC). It has been difficult to justify this rapid thermalization in
first-principle calculations based on perturbation theory or the color glass
condensate picture. Here, we address the related question of the decoherence of
the gluon field, which is a necessary component of thermalization. We present a
simplified leading-order computation of the decoherence time of a gluon
ensemble subject to an incoming flux of Weizsacker-Williams gluons. We also
discuss the entropy produced during the decoherence process and its relation to
the entropy in the final state which has been measured experimentally.Comment: 8 pages, 3 figure
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